Rapid Selection of Halophilic Streptomonospora Strains by PCR-SSCP
- VernacularTitle:PCR-SSCP技术在嗜盐放线菌链单孢菌属快速筛选中的应用
- Author:
Man CAI
;
Xiao-Yang ZHI
;
Jin-Yuan WU
;
Shu-Kun TANG
;
Wen-Jun LI
;
- Publication Type:Journal Article
- Keywords:
PCR-SSCP, Streptomonospora, Rapid selection
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To improve the efficiency of halophilic actinobacteria screening and carry out the rapid selection of targeted strains, we tested 34 strains of Streptomonospora by PCR-single strand conformation polymorphism analysis (PCR-SSCP) based on genus-specific primers for the PCR identification. This approach employs PCR with two pairs of primers located in the 16S rRNA sequence flanking two variable region, then build clustering tree according as SSCP data. Synchronously, we sequenced all the 16S rRNA partial sequences for these strains to verify them. The results showed that the PCR-SSCP analysis was an efficient, easy-to-handle and economic method for rapid selection of halophilic actinobacteria resources.
