Isolation and Identification of BLN-2 Producing Poly-?-Glutamic Acid and Studies on Its Solid-state Fermentation
- VernacularTitle:?-聚谷氨酸产生菌BLN-2的分离鉴定及固体发酵条件初探
- Author:
Jing FENG
;
Qing-Shan SHI
;
Xiu-Lin SHU
;
Xiao-Ping LIN
;
You-Sheng OUYANG
;
Yi-Ben CHEN
;
- Publication Type:Journal Article
- Keywords:
Poly-?-glutamic acid (?-PGA), Bacillus subtilis, Isolation, Identification, Solid-state fermentation
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
A poly-?-glutamic acid producing strain--BLN-2, was isolated from the soybean products. According to the biochemical characteristics and 16S rRNA, the strain was identified as Bacillus subtilis. Using soybeans as culture, the solid-state fermentation conditions of BLN-2 have been studied. The results showed that the optimal carbon and nitrogen sources of BLN-2 were glucose, fructose, NaNO3 and KNO3, respectively. The orthrogonal experiments showed, when the final concentration of the fructose which was added to the soybean culture was 0.5%, the glucose, NaNO3 and KNO3 final concentraion were 2.0%, the production of ?-PGA was the highest--89.05 g/kg. It is 48.42% higher than other comparable soybean medium under the same conditions.
