Effect of Carbon Dioxide Pneumoperitoneum on Inflammatory Response and Bacterial Translocation in Rabbits with Intra-abdominal Infection
- VernacularTitle:二氧化碳气腹对腹内感染炎症反应及细菌易位的影响
- Author:
Gang WANG
;
Rongde WU
;
Qihai YU
;
- Publication Type:Journal Article
- Keywords:
Pneumoperitoneum;
Laparoscopy;
Bacterial translocation;
C-reactive protein
- From:Chinese Journal of Minimally Invasive Surgery
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of CO_2 pneumoperitoneum on animals with intra-abdominal infection.Methods A total of 60 New Zealand white rabbits were used in this study.The animals were divided into two groups (30 in each),and injected with Escherichia coli or bacteroides fragilis into the abdominal cavity to establish models of intra-abdominal infection.In each group, the rabbits were subdivided into laparotomy,pneumoperitoneum,and control subgroups.Before the injection,the peripheral blood of the rabbits in each group was obtained to determine the WBC count and level of C-reactive protein (CRP,by ELISA).After the operation,the tests were repeated for 4 times at days 1,2,4,7 respectively.Afterwards,the animals were killed,and the viscera were harvested for aerobic and anaerobic culture.Results In each of the two groups,the WBC count in the laparotomy subgroup were significantly higher than that in the pneumoperitoneum subgroup at day 1 (P
