VSMCs Proliferation and Mechanism of Signal Transduction by Angiotensin Ⅱ Type 1 Receptor Autoantibodies Mediated from Hypertensive Patients
- VernacularTitle:原发性高血压患者中抗血管紧张素Ⅱ1型受体自身抗体所致大鼠主动脉平滑肌细胞增殖及胞内信号研究
- Author:
Yan-Xiang SUN
;
Yu-Hua LIAO
;
Yu-Miao WEI
;
Hai-Yan ZHANG
;
Min WANG
;
Feng ZHU
;
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ type 1 receptor;
Autoantibody;
Cell proliferation;
Signaling mechanism
- From:
Chinese Journal of Hypertension
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The autoantibodies against angiotensin Ⅱ type 1 receptor(AT_1 RAb)have been dis- covered in the patients with malignant hypertensive and preeclampsia,this autoantiboies(AT_1-AA)have an ago- nist-like activity effect similar to angiotensin Ⅱ(Ang Ⅱ).This study aimed at investigation the effect of Ang Ⅱ agonist-like activity by AT_1-AA on VSMCs proliferation was obtained from essential hypertensive patients. Methods VSMCs were cultured from aorta of WKY rats.The hypertensive patients" serum was purified by am- monium sulfate precipitation and affinity chromatography.The effect on VSMC proliferation this autoantilody was determined by BrdU incorporation.Total protein and the expression of phosphorylation JAK-STAT were assessed by Western blotting.Results AT_1RAb caused a significant increase in BrdU incorporation similar to Ang Ⅱ during 0-24 h reaching peak value at 12 h.The A value of in 450 nm was higher in AT_1RAb group (0.236?0.012)than AG490+AT_1RAb group(0.176?0.009),Losartan+AT_1RAb groups(0.119?0.006) and Serum Free group(0.127?0.006)(P
