Development and Clinical Application of RT-PCR Differential Diagnosis Method for High Virulent Porcine Reproductive and Respiratory Syndrome
- VernacularTitle:高致病性猪繁殖与呼吸综合征病RT-PCR快速鉴别诊断方法的建立与临床应用
- Author:
Zhong-Hua LIU
;
Xing-Long YU
;
Run-Cheng LI
;
Ze-Bin HUANG
;
Li-Shan LIAO
;
Xia BAI
;
Jing LI
;
Wei-Jun XIANG
;
Zhen-Nan WANG
;
Jian DING
;
- Publication Type:Journal Article
- Keywords:
High virulent porcine reproductive and respiratory syndrome virus, RT-PCR, Differential diagnosis
- From:
Microbiology
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the deletion information of high virulent PRRSV genome, 3 oligonucleotide primer were designed and synthesized. Specific and sensitive reverse transcription-PCR (RT-PCR) assays were de-veloped for the detection of high virulent PRRSV. The sensitivity and specificity of RT-PCR assays were evaluated, the results showing that the detection limit of the assay was found to be 0.265 pg of tissue total RNA, and the protocol have no cross-reaction with classical swine fever virus, porcine circovirus type 2,pseudorabies virus, streptococcus, haemophilus parasuis and Escherichia coli. Then 36 cell cultures, two PRRSV live vaccine strains and 184 clinical specimens from 52 farms were tested. Five PRRSV field iso-lates were the high virulent PRRSV; two PRRSV live vaccine strains from normal PRRSV, and 123 speci-mens from 42 farmer were positive (only 1 specimen was normal PRRSV). This RT-PCR method proved to be accurate differential diagnosis of the high virulent PRRSV and normal PRRSV with the characteristics of rapidity, sensitivity and specificity, and has a strong clinical relevance.
