Isolation and Identification of Aeromonas hydrophila Strain X1 from Acipenser baerii and Its Antibiotic Sensitivity
- VernacularTitle:鲟源致病性嗜水气单胞菌X1的分离鉴定与药敏特性研究
- Author:
Yuan-Yuan LI
;
Hai-Peng CAO
;
Shan HE
;
Xian-Le YANG
;
- Publication Type:Journal Article
- Keywords:
Acipenser baerii, Aeromonas hydrophila, Identification, Antibiotic sensitivity
- From:
Microbiology
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
A pathogenic bacterial strain X1 was isolated from Siberian sturgeon (Acipenser baerii) suffering with bacterial septicemia. The 50% lethal concentration (LC50) of strain X1 was 5.62?105 CFU/mL, which showed that strain X1 was rather strong virulent to Acipenser baerii. Strain X1 was gram negative and 1.0 ?m～1.2 ?m ? 2.1 ?m～2.4 ?m in size with peritrichous flagella, and had ?-hemolytic activity on rabbit blood agar. By means of ATB expression identification and 16S rDNA sequence analysis, strain X1 was identified as Aeromonas hydrophila (locus number: EU669667), which was the closest relative to Aeromo-nas hydrophila strain ATCC35654 (locus number: X74676.1) with 99% homology. In addition, strain X1 was highly sensitive to cefoperazone and cravit, and intermediately sensitive to ten kinds of antibiotics in-cluding tobramycin, norfloxacin, sulperazone, kanamycin, gentamycin, fortum, vancomycin, neomycina, polymyxin B and lomefloxacin.
