Cloning and Expression Analysis of two Endosperm Promoters in Rice
- VernacularTitle:两个水稻胚乳启动子的克隆及表达分析
- Author:
Yong-Lan CUI
;
Xiao-Li ZHONG
;
Yong-Ming ZHANG
;
Zhong-Nan YANG
;
- Publication Type:Journal Article
- Keywords:
Rice (Oryza sativa L) Endosperm promoter GUS reporter
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
The cloning of promoter is important for studying the genetic engineering and the regulation of gene expression in plants. Two promoters Os772 and Os359, which are predicted to be highly expressed in the endosperm of rice from the EST database were cloned. After construction of the Os772∶∶GUS and Os359∶∶GUS expression vectors, they were transformed into rice. X-Gluc staining of transgenic plants showed that Os772 and Os359 can promote GUS gene expression in matured endosperm but not in root, stem, leaf and flower. This result indicates Os772 and Os359 are two rice endosperm-specific promoters.
