Enhancing effect of CpG on sensitivity of Lewis lung cancer to X-ray radiation in mice
- VernacularTitle:CpG对X线放射治疗Lewis肺癌小鼠移植瘤的增敏效应
- Author:
Su-Juan YUAN
;
Tian-Kui QIAO
;
Ji-Min SHI
;
Hui-Zhong HE
;
- Publication Type:Journal Article
- Keywords:
cytosine-phosphate-guanine oligodeoxynueleotide;
Lewis lung cancer;
tumor growth delay;
the sensitization enhancement ratio;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the role of cytosine-phosphate-guanine oligodeoxynucleotide(CpG ODN)in enhan- cing the radiosensitivity to X-ray in mouse with Lewis lung cancer.Methods: The tumor-bearing mouse model was in- duced by injevting Lewis lung cancer cells into the right infra-axillary dermis.Thirty-two C57BL/6J mice were evenly ran- domized into 4 groups.Group A: the control group;Group B: the X-Ray radiation group;Group C: the CpG group; Group D: the CpG plus X-Ray radiation group.Group B was treated with X-Ray radiation only(3 Gy/F,on day 1,3,5, 8,10,and 12;the total dose was 18 Gy);group C was administered with CpG ODN 0.05 mg on day 1,3,5,8,10, and 12;group D was administered with CpG ODN 6h before X-ray radiation.The tumor growth and tumor growth delay (TGD)were observed in all groups.Meanwhile,the pathological change of the tumor tissue was observed with H-E staining method and the apoptosis of tumor cells were examined with the method of TUNEL.Results: The Lewis hmg cancer-bearing model was successfolly established in mice.The tumor volumes of the treatment groups were smaller than that in lhe control group(P
