Inhibitory effect of adenovirus mediated VEGF-siRNA on transplanted osteosarcoma in nude mice
- VernacularTitle:腺病毒介导人VEGF-siRNA对裸鼠移植骨肉瘤的抑制作用
- Author:
You-Shui GAO
;
Jiong MEI
;
Tian-Lang TONG
;
Mu HU
;
Hua-Ming XUE
;
Xuan-Song CAI
;
- Publication Type:Journal Article
- Keywords:
adenovirus;
vascular endothelial growth factor(VEGF);
osteosarcoma;
RNA interference(RNAi);
gene therapy;
antiangiogenic therapy
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect of adenovirus-mediated VEGF-siRNA on transplanted osteo- sarcoma in nude mice.Methods: VEGF-siRNA gene was cloned into the genome of replication-deficient adenovirus to construct Ad-VEGF-siRNA;the latter was then used to infect osteosarcoma MG63 cell line in vitro;and the expression of VEGF gene was detected by RT-PCR.Osteosarcoma transplantation model was established in nude mice;VEGF expression in tumor tissue was analyzed and the inhibitory effect on tumor growth and lung metastasis were also observed.Results: The recombinant adenovirus vector Ad-VEGF-siRNA was successfully constructed.In vivo and in vitro experiment both showed that Ad-VEGF-siRNA significantly downregulated VEGF expression in MG63 cells and transplanted tumor tissue. It was found that Ad-VEGF-siRNA significantly inhibited transplanted osteosarcoma growth(P
