Recombinant mutant human tumor necrosis factor enhances anti-angiogenesis effect of cisplatin on Lewis lung carcinoma in mice
- VernacularTitle:rmh-TNF协同顺铂抗小鼠Lewis肺癌血管生成的作用
- Author:
Su-Ju WEI
;
Jiang-Lian YOU
;
Wen-Feng HAN
;
Yi-Bing LIU
;
- Publication Type:Journal Article
- Keywords:
recombinant mutant human tumor necrosis factor;
cisplatin;
Lewis lung carcinoma;
synergism;
antiagniogenesis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the role of rmh-TNF in enhancing the anti-angiogenesis effect of cisplatin on Lewis lung carcinoma in the mice.Methods: Lewis lung carcinoma model was established in C57BL/6 mice.Sixty model mice were randomly divided into 4 groups: control group,rmh-TNF group(1500000 U/kg),cisplatin group(6.15 mg/kg), and rmh-TNF plus cisplatin group.Twelve days after implantation of cancer cells,different drugs were injected intra- tumorallv for 3d.The expression of hypoxia inducible factor-1?(HIF-1?)gene in the tumor was identified by RT-PCR. Immunohistochemistry(IHC)image analysis was performed to determine the vascular endothelial growth factor(VEGF) and kinase domain region receptor(KDR)expression and the microvessel density(MVD).Expression of matrix metallo- proteinase-2(MMP-2)was detected by flow cytometry.Results: The MVD values in the control group,the rmh-TNF group,the DDP group and the combination group were(24.76?1.28),(18.95?1.22),(19.53?1.15),(10.43?1.05),respectively,with those of the rmh-TNF and DDP groups significantly lower than that of the control group and higher than that of the combination group(all P
