Inhibitory effect of artesunate on human esophageal carcinoma cells and the possible mechanism
- VernacularTitle:青蒿琥酯对人食管癌细胞的抑制作用及其可能的机制
- Author:
Liang LIU
;
Jing WANG
;
Jian-Wen GUO
;
Jiang-Hui LIU
;
Lian-Fu ZUO
;
- Publication Type:Journal Article
- Keywords:
artesunate;
esophageal squamous cell carcinoma;
cell division cycle 25A(CDC25A);
Smad3;
transforming growth factor-?;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the inhibitory effect of artesunate(Art)on human esophageal carcinoma cells and to study the related mechanism.Methods: Nude mice were inoculated with Eca109 cells subcutaneously on the left upper limbs to establish esophageal carcinoma model.The model mice were divided into 5 groups: first group received 100 mg/kg Art,second group 200 mg/kg Art,third group 300 mg/kg Art,forth group 3 mg/kg cisplatin(DDP),and the fifth group received normal saline.Mass and volume changes of transplant tumors in different groups were observed.Flow cytometry was used to detect the cell cycle,apoptosis,and the expression of CDC25A protein,Smad3 protein and TGF-?protein in the transplanted tumors in mouse model.RT-PCR was used to detect the expression of CDC25A,Smad3 and TGF-?mRNA in the transplanted tumors.Results: Nude mouse model bearing human esophageal carcinoma was success- fully created.Compared with the control group,the volume and mass of transplant tumors in Art groups were significantly smaller(P
