Study on Heterologous Expression of Penicillinase Gene and the Penicillinase Degrading Residual Penicillin in Milk
- VernacularTitle:青霉素酶基因异源表达及该酶分解牛奶中残留青霉素的研究
- Author:
Hong-Kun ZHAO
;
Lian-Xiang DU
;
Yu LI
;
Xiao-Juan WANG
;
Fu-Ping LU
;
- Publication Type:Journal Article
- Keywords:
Penicillinase,Heterologous expression,Purification,Immobilized penicillinase
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To obtain a number of penicillinases and degrade penicillin in milk by using the penicillinases,the gene encoding penicillinase was amplified by PCR from Bacillus cereus ATCC10987,cloned into pET28a(+) ,transformed into E. coli BL21;analysis of SDS-PAGE and penicillinase activity of the recombinant protein were done under induction of IPTG and the result showed that the maximum penicillinase activity reached 480 U/mL;the purity of penicillinase purified by Ni2+ Purification System was more than 90%;the immobilized penicillinases were obtained by sodium periodate method and the residual quantity of penicillin in milk(containing 0.5 U penicillin G/mL) was less than 4 ppb after degraded by the immobilized penicillinase.
