Immobilization of ?-glucosidase with Nano SiO_2 and Its Application in Soybean Isoflavone Hydrolysis in Two-phase-system
- VernacularTitle:纳米SiO_2固定化?-葡萄糖苷酶及其在双相体系中水解大豆异黄酮的工艺研究
- Author:
Zhe HUANG
;
Tao ZHANG
;
Zhang-Lin LIN
;
- Publication Type:Journal Article
- Keywords:
Nano SiO_2 Immobilized enzyme Two phase system Soybean isoflavone Enzymatic hydrolysis
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Nano materials are popular with its interesting properties. Large specific surface area, high mechanical strength, good absorbability make them good potencial carriers of catalyst. With the help of chemical modification, nano SiO2 particals were used as carrier to immobilize ?-glucosidase with covalent binding. It is found that the load of nano particals can reach 1/8 of its weight, and the recovered activity of enzyme is 70%. The resistence of immobilized enzyme to organic solvent wase improved obviously, which was then used in Ethyl Acetate-Water-two-phase-system to hydrolyzed soybean isoflavones.
