Separating and Studying of the Aerobic Denitrifying Bacteria from Biofilter
- VernacularTitle:生物滤塔体系好氧反硝化菌的分离鉴定与特性研究
- Author:
Li-Rong FAN
;
Shao-Bin HUANG
;
Jun YANG
;
Yong-Qing ZHANG
;
- Publication Type:Journal Article
- Keywords:
Biofilter, Aerobic denitrifying bacteria, Bacteria screening, Bacteria identification
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Four strains of aerobic denitrifying bacteria were isolated from biomembrane of the laboratory biofilter by selective medium. The denitrifying rates of these four strains were found more than 80% under aerobic condition for 40 hours. The denitrifying rate of A1 was highest, which was 99.05%. When measuring the course of nitrogen element changing, it is found that they accumulated nitrite expect the strain A1. The strain A1 was gram positive and spherical. It is identified as Pseudomonas putida based on its biochemical and morphological characters and phylogenetic analysis of 16S rDNA sequences(genbank accession NO.DQ836052.1). For the strain A1, the optimum beginning pH was 7.0 around, and the optimum tempera- ture was 30 ℃ around, The change of DO did not influence the effect of denitrification when it was more than 2.0 mg/L.
