Numerical Taxonomy and 16S rDNA PCR-RFLP of Rhizobial Strains Isolated from Psoralea corylifolia etc
- VernacularTitle:分离自补骨脂等宿主根瘤的24株菌的数值分类和16S rDNA PCR-RFLP研究
- Author:
Yun-Jie HAO
;
Meng-Zhao WANG
;
Lei LIU
;
Su-Zhen HAN
;
- Publication Type:Journal Article
- Keywords:
Rhizobium, Numerical taxonomy, 16S rDNA PCR-RFLP
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
24 strains obtained from root nodules of Psoralea corylifolia, Pueraria lobata, and Campylotropis macrocarpa of Yunnan province were studied with numerical taxonomy and 16S rDNA PCR-RFLP. Results of numerical taxonomy indicated that all strains included 10 reference strains were divided into 3 groups at 84% similarity. Group III is an unknown group with no reference strains. Group I is slow-growing kind, and group II fast and middle-slow-grower. The dendrogram derived from 16S rDNA PCR-RFLP showed that all strains divided into five phylogenetic branches at the similarity of 70%. They are branches I and V with no reference strains, Agrobacterium-Sinorhizobium-Rhizobium, Mesorhizobium and Bradyrhizobium. Not all results of numerical taxonomy are accord with 16S rDNA PCR-RFLP, and 2 strains at the same group with A. tumefaciens IAM13129T.
