The Duplex PCR Detection of Transgenic Maize Transformed by Rice Rough Dwarf Virus Movement Protein Defective Gene
- VernacularTitle:转水稻粗缩病毒运动蛋白缺陷型基因玉米的二重PCR检测
- Author:
Jian-Zhong DU
;
Yi SUN
;
Yao-Shan HAO
;
Jian HE
;
- Publication Type:Journal Article
- Keywords:
Rice rough dwarf virus movement protein defective(RDV MP-)gene Maize Duplex PCR amplification False negaive
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A simple and effective method for the duplex PCR detection was developed by using sequences of exogenous gene(RDV MP-)and endogenous gene(Zein)as templates for PCR amplification.The results of routine PCR amplification for RDV MP-gene in transgenic maize suggested that RDV MP-gene can stably inheritate in transgenic plants and their progenies;The duplex PCR detection of all negative and part positive samples that obtained by routine PCR amplification confirmed that above negative results were exact,also showed that the quality of extracted DNA can meet the need of PCR amplification.The error ratio of negative samples was 1.4%.The method used in this study was simple and credible and can be used to detect transgenic plants and their products.
