The Genetic Stability of Replicating HBV Transgenic Mice
- VernacularTitle:复制型HBV转基因小鼠遗传稳定性研究
- Author:
Xiang-Ping KONG
;
Qing-Zhou WU
;
Xian-Rong LUO
;
Lian-Mei HU
;
Xiu-Mei LI
;
Xue-Rui YI
;
Ming-Hua TONG
;
Jun-Hui ZHOU
;
Guang-Ze LIU
- Publication Type:Journal Article
- Keywords:
HBV transgenic animal Breeding Replication Stability
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the genetic stability of HBV gene in transgenic mice.Methods:HBV transgenic mice were bred by backcross and double cross.The HBV gene expression and replication were studied with real-time PCR,ELISA and chemiluminescence.Results:The HBV transgenic mice have stably bred to 23rd generation.The serum HBsAg level is 4122.31?2044.74IU/ml;The rate of HBV transgenic mice whose serum HBV DNA reach 104~106copies/ml was 93.93%.The HBV replication and expression were improved markedly.There is no difference between male and female mice about serum HBsAg level.Conclusion:After breeding the HBV gene was expressed stably with high-level in transgenic mice.
