Retroviral Expression System and Its Application in Efficient Foreign Gene Expression
- VernacularTitle:逆转录病毒表达系统及其在外源蛋白高效表达中的应用
- Author:
Jian XU
;
Shi-Chong LI
;
Zhao-Lie CHEN
;
- Publication Type:Journal Article
- Keywords:
Retroviral expression system Vesicular stomatitis virus G protein Virus titer Recombinant protein High expression
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Retroviral expression system which consists of retroviral vector,envelop protein vector and packaging cell line is an efficient expression system for recombinant protein.It has great potential in gene therapy and biopharmacy.Transcriptional active genome regions are the preferred targets for retrovirus integration.Furthermore,VSV-G protein enables this system a broader host range and makes virus integration more efficient.After infection of high-titer virus,high production clones can be selected through simple screening.So far,the research on retrovirus expression system has developed into application in bio-pharmacy industry.Here the composition of this system and the mechanism of virus transduction and summarize the application and prospect of retroviral expression system are introduced.
