Effects of exercise with different intensity on glucose transport protein contents in the skeletal muscle of diabetes rats
- VernacularTitle:糖尿病大鼠骨骼肌细胞膜葡葡糖转运蛋白4含量在不同强度运动状态下的变化
- Author:
Yong-Chun LI
;
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Exercise affects glucose transport 4(Glut4) protein levels in the skeletal muscle, but different intensions of exercise effects are unknown to people. OBJECTIVE: To explore effects of different intensity exercise on Glut4 protein expression in the skeletal muscle of rats. DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Central Laboratory of Liaoning Normal University from September to October 2007. MATERIALS: A total of 60 healthy SD rats weighing (200?16) g of both genders were used in the following study. METHODS: Rat models of diabetes mellitus were created and divided into six groups: a normal control group, a diabetes mellitus control group and a diabetes mellitus group including 15 m/min group, 20 m/min group, 25 m/min group and 30 m/min group. Each group consisted of 10 rats. The exercise groups did the running exercise each day for 50 minutes, 6 days in a week, lasting for 6 weeks. MAIN OUTCOME MEASURES: Western bolt analysis was used to determine Glut4 protein on cell membrane of the skeletal muscle. Different intensions of exercise effects on body weight, blood glucose and insulin levels were observed in rats from each group. RESULTS: Compared with the diabetes mellitus control group, Glut4 protein contents enhanced up to 44.2% and 48% (P
