The Role of Transforming Growth Factor-?_1 in the Proliferation of Cardiac Fibroblasts Induced by Chymase
- VernacularTitle:糜酶介导心脏成纤维细胞增殖中转化生长因子?_1的作用
- Author:
Xiao-Yan ZHAO
;
Lian-You ZHAO
;
Qiang-Sun ZHENG
;
Xiao-Long LU
;
Yan-Ping HE
;
- Publication Type:Journal Article
- Keywords:
Chymase;
Cardiac fibroblasts;
Proliferation;
Transforming growth factor-?_1
- From:
Chinese Journal of Hypertension
2007;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of chymase on the proliferation of rat cardiac fibroblasts (CFs) and the role of transforming growth factor-?1 (TGF-?_1).Methods Cultured CFs of neonatal SD rats were isolated by trypsinization.Cell number and DNA synthesis were evaluated by MTT assay (A_(490) value) and [~3H]-deoxythy- midine [~3H]-TdR incorporation.The mRNA expression of TGF-?_1 in CFs was determined by RT-PCR.Results Chymase increased CFs numbers and [~3H]-TdR incorporation in a dose-dependent manner.The A_(490) value of CFs stimulated by 15,30 and 60 ng/mL chymase was 0.263?0.033,0.348?0.031 and 0.387?0.026,respectively, which were all significantly higher than that of control (0.201?0.019,P
