Analysis of Signal Peptide Fragment of Canine Distemper Virus Wild-type Strain Fusion Protein
- VernacularTitle:犬瘟热病毒临床流行野毒株F蛋白信号肽区分析
- Author:
Feng-Xue WANG
;
Xi-Jun YAN
;
Xiu-Li CHAI
;
Wei WU
;
Xi-Qun SHAO
;
Guo-Liang LUO
;
Hai-Ling ZHANG
;
Li YI
;
Jian-Jun ZHAO
- Publication Type:Journal Article
- Keywords:
Canine distemper virus,Fusion protein,Signal peptide
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A pairs of primers were designed according to the fusion protein(F)gene sequences of canine distemper virus(CDV)in GenBank.A 369 bp fragment aimed signal peptide fragment of F gene was amplified.The PCR products from viscera samples,blood,urine of fur animals including foxes,minks and raccoon dogs,which collected in the years 2005-2007,were cloned to pMD18-T Vector and sequenced.We obtained 13 positive signal peptide fragments from wild-type strains.The results indicated there was obviously genetic diversity between the wild-type strains and CDV3 and other vaccine strains.The homology with CDV3 is 80.7%-83.2%in nucleotide,and 64.8%-71.3%in amino acid.The analysis for the hydrophobic regions indicated the function of signal peptide fragment may be changed.This study can offer aca- demic data to research of CDV genetic variation and epidemiology.
