Cloning of Ginseng ? AS Gene and the Construction of Its Antisense Plant Expression Vector
- VernacularTitle:人参皂苷合成相关? AS基因的克隆及其反义植物表达载体的建立
- Author:
Shou-Jing ZHAO
;
Chun-Xi HOU
;
Yan-Long LIANG
;
Jian XUE
;
Jian-Hua WANG
;
- Publication Type:Journal Article
- Keywords:
Ginseng ?-amyrin synthase Antisense plant expression vector
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
RT-PCR amplification of ginseng ?-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method. Sequence analysis of this gene revealed that its sequence was consistent with the sequence of a previously reported ginseng ?-amyrin synthase gene (GenBank No. AB009030). This gene was inserted into pMD-119T simple vector and transformed into E.coli DH5?. Furthermore antisense plant expression vector of this gene was constructed using the pBI121 vector, laying foundation for studies on antisense regulation of ginseng ?-amyrin synthase gene.
