Study of Expression of Pneumococcal Surface Protein and Immunogenicity
- VernacularTitle:肺炎球菌表面蛋白的克隆表达与免疫原性研究
- Author:
Qian-Ying CAI
;
Liang FANG
;
Jin-Zhong HUANG
;
Hai-Ying LIN
;
Yang-Hao GUO
;
Chun MENG
;
- Publication Type:Journal Article
- Keywords:
PspA gene PsaA gene Immunogenicity Prokaryotic expression
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The specific fragment of Pneumococcal surface protein A(PspA)and Pneumococcal Surface Adhesin A(PsaA)gene was amplified by PCR from Streptococcus pneumonia 5 and Streptococcus pneumonia 19.The amplified fragnent of PspA and PsaA gene was ligated into pET-27b(+)vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA.Induced by IPTG,the expression level was as high as 75 % of the total disolube protein.The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E.coli BL 21,which showed the good immunogenicity and a broadly cross reactivity with the other serotypes.
