Inhibition of Hyperplasia of Vascular Smooth Muscle Cell by RNA Interference of The Plasmids of Cysteine-rich61
- VernacularTitle:富含半胱氨酸蛋白61 RNA干扰质粒抑制血管平滑肌细胞的增殖
- Author:
Peng FU
;
Tie-Sheng NIU
;
Ying-Xian SUN
;
- Publication Type:Journal Article
- Keywords:
RNA Interference;
Vascular smooth muscle cell;
Cysteine-rich 61;
Cell Proliferation;
Gene Transfection
- From:
Chinese Journal of Hypertension
2007;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of RNA interference plasmids of cysteine-rich 61(Cyr61) on hyperplasia of vascular smooth muscle cell (VSMC) in rats.Methods The plasmids containing the short hairpin RNA (shRNA) of Cyr61 were constructed.Expression of Cyr61 mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.The hyperplasia of VSMC was assessed by MTT.DNA synthesis was measured by incorporating ~3H-TDR.Plasmid construction was confirmed by DNA sequencing.Results PCyr61-shRNA transfection significantly decreased the level of mRNA and protein of Cyr61 in VSMC.The cell number,optical density and concentration of DNA in pCyr61-shRNA group were significant decreased(P
