Telmisartan Prevent Oxidative Injuries in Endothelial Cells Induced by High Glucose
- VernacularTitle:替米沙坦减轻高糖引起的人血管内皮细胞损伤
- Author:
Jun HUANG
;
Jing XIAO
;
Lian-Man HE
;
Hai-Yan QIN
;
De-Qiang LI
;
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cell;
Telmisartan;
Malondialdehyde;
Super oxide dismutase;
Peroxisome proliferator activated receptors ?
- From:
Chinese Journal of Hypertension
2007;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of telmisartan on the oxidative stress induced by high glucose in human umbilical vein endothelial cell (HUVEC) in vitro.Methods HUVEC were cocultured with telmisartan (1?10~(-6) mol/L) and various concentration of glucose(5,30 mmol/L) for 0,12,24,36,48 h respectively. The level of MDA in the supernatants of cultured endothelial cells was measured by thiobarbituric acid test,SOD was determined by xanthine oxidase test.The protein expression of peroxisome proliferator activated receptors ? (PPAR-?) in HUVEC 24 h was assessed by Western blot after treatments.Results High glucose significantly increase the levels of MDA (before:1.2?0.06 vs after:1.6?0.1 mmol/mL,P
