Aspirin Inhibits Fractalkine Expression Stimulated by Tumor Necrosis Factor ? in Human Umbilical Vein Endothelial Cells
- VernacularTitle:阿司匹林抑制肿瘤坏死因子?诱导的人脐静脉内皮细胞分形趋化因子表达
- Author:
Hong LIU
;
De-Qian JIANG
;
She-Bing ZHANG
;
- Publication Type:Journal Article
- Keywords:
Fractalkine;
Aspirin;
Human umbilical vein endothelial cells;
Nuclear factor-kB
- From:
Chinese Journal of Hypertension
2007;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Background Fractalkine is involved in the impairment of endothelium by mediating inflammatory cell chemotaxis.Aspirin inhibites many kinds of cytokine expression.Objective To investigate the effect of aspirin on tumor necrosis factor ?(TNF-?) stimulated fractalkine expression in human umbilical vein endothelial cells (HUVEC) and its mechanism.Methods HUVEC were grouped as follows:control group;TNF-?-stimu- lated;TNF-?+NS-398;TNF-?+PDTC and TNF plus various concentration of aspirin (0.02,0.2,1.5 mmol/L). The level of mRNA and protein expression of fractalkine and nuclear factor(NF)-kB p65 was determined by RT- PCR and Western blot.Results 1)Fractalkine mRNA and protein level was increased after 4 ?g/L TNF-? stim- ulation in HUVEC(both P
