Integration and Expression of BGL1 Gene from Saccharomy-copsis fibuligera in Industrial Saccharomyces cerevisiae
- VernacularTitle:扣囊复膜孢酵母BGL1基因在工业酒精酵母中的整合表达
- Author:
Liang ZHANG
;
Yan ZHOU
;
Gui-Yang SHI
;
- Publication Type:Journal Article
- Keywords:
Industrial yeast, ?-glucosidase, Homologous integrated, Hygromycin B
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The recombinant plasmid pPIC-gpd-bgl-hyg was constructed, which contained GPD2 promotor and terminator from industrial yeasts Saccharomyces cerevisiae, ?-glucosidase gene (BGL1) from Sac-charomycopsis fibuligera and hyg from hygromycin as the selected marker. With the yeast’s high efficiency of homologous integrated, the BGL1 gene was successfully integrated into industrial yeasts S. cerevisiae. The recombined yeast could grow on the cultures with the cellobiose as a sole carbon source, and the ?-glucosidase activity achieved 0.764 U/mL after 48 hours’ cultivation. In the experiments of VHG ethanol fermentation, the cellobiose concentration in broth of recombined yeast was 80% lower than that of indus-trial yeast.
