Metabolic Flux Analysis of L-Arginine Fermentation in Corynebacterium glutamicum
- VernacularTitle:L-精氨酸生物合成的代谢流量分析
- Author:
Jin-Wei ZHU
;
Qing-Shan CHEN
;
Wei-Guo ZHANG
;
- Publication Type:Journal Article
- Keywords:
L-Arginine, Corynebacterium glutamicum, Metabolic flux analysis
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, metabolic networks of the Corynebacterium glutamicum GWY020 and the two de-rivatives carrying additional mutations HUI821and GUI089 were established and modified. The concentra-tions of extra-cellular metabolites were determined under sub-steady-state (50 h~52 h) of the batch culture. The metabolic flux distribution maps of the three strains were obtained, compared and analyzed. These re-sults indicate that the introduction of analog supersensitive marker or analog resistant marker skew the metabolic flux towards the formation of L-Arginine. This study revealed the usefulness of the metabolic flux analysis as a tool for verification of existing production strains. The analysis may play an important role in helping us to rationally re-design metabolism for further improvement of fermentation process.
