T_RFLP Technique and Its Application on Community Analysis of Nitrifying Bacteria
- VernacularTitle:T_RFLP技术及其在硝化细菌群落分析中的应用
- Author:
Jian-Fei LUO
;
Wei-Tie LIN
;
Jie REN
;
Hua-Ping CUI
;
- Publication Type:Journal Article
- Keywords:
T_RFLP, Community analysis, Nitrifying bacteria
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Terminal restriction fragment length polymorphism (T_RFLP) analysis is a culture- independent approach for analyzing microbial community in environment. It bases on PCR technology, and its process includes DNA extraction of environmental samples, amplification of genes encoding the 16S rRNA, 18S rRNA or enzymes with fluorescently labeled primers, the restriction enzyme digestion of PCR products, cap- illary electrophoresis and the analysis of T_RFLP profile. It has been proved to be powerful applied on mi- crobial community in environment since developed in 1997. Currently, T_RFLP rarely applied in China, and it has no applications on microbial community analysis of nitrifying bacteria. In this article, the fundamental principle of this technique and the recent applications of T_RFLP on microbial community are summarized; in addition, it illustrates the confinements of conventional culture-dependent of nitrifying bacteria and the foreground of T_RFLP applying on microbial community structure analysis of nitrifying bacteria.
