A Modified Method for Isolating DNA from Fungus
- VernacularTitle:一种改进的丝状真菌DNA提取方法
- Author:
Ying-Hui ZHANG
;
Dong-Sheng WEI
;
Lai-Jun XING
;
Ming-Chun LI
;
- Publication Type:Journal Article
- Keywords:
Extraction, CTAB, Glass beads, Thawing and freezing test of liquid nitrogen
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Genomic DNA of two fungi Thamnidium elegans and Umbelopsis isabellina were extracted with an amended Cetyltrimethyl Ammonium Bromide (CTAB) method. This modified method uses repeated freezing in liquid nitrogen and thawing with combination of shocking with glass beads to replace of the tra- ditional method. Quality and concentration of DNA extracted by the modified methodwere tested. Compared with the traditional method, higher yield and purity of genomic DNA were obtained with less amount ofmy- celium. The result indicted that this is a simple and highly efficient method, which is suitable to treat many samples at one time and for basic molecular experiments, such as restriction endonuclease reaction and PCR.
