Research on Separation Characteristics of the Extracellular Algae-lysing Components from Three Algae-lysing Bacteria
- VernacularTitle:三株溶藻细菌胞外溶藻活性物质若干分离特性的研究
- Author:
Yan LI
;
Wei-Bin PAN
;
Li-Li YANG
;
- Publication Type:Journal Article
- Keywords:
Eutrophication,Algae-lysing bacteria,Extracellular algae-lysing components,Separation
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Three algae-lysing bacteria (L7、L8、L18) have been isolated. We used starch medium,which has no passive effect on growth of the Anabaena flos-aquae as cutivate medium. After being cultivated for 4d on a reciprocal shaker at 30℃ and a speed of 100 r/min,the cultivate broth were centrifugated at a speed of 4000 r/min for 20min、filtrated with 0.22 ?m membrane,and condensed by vacuum rotary evaporator at 65℃. The sterile condensed bacteria-free filtrate obtained are dialysed,sedimentated by ethanol,extracted by organic solvents,absorbed by activated carbon. The molecular weight of the extracellular algae-lysing components of L7 are less than 3.5 kD,while the molecular weight of extracellular algae-lysing components of L8 and L18 are between 3.5 kD～7 kD; ethanol can not separate extracellular algae-lysing components from other componets efficiently; carbon tetrachloride can separate the extracellular algae-lysing components of L7 into water-layer efficiently,while petroleum ether can separate the extracellular algae-lysing components of L8 and L18 into water-layer efficiently,the ex-tracellular algae-lysing
