Inhibition of CaMKⅡ? Expression by RNA Interference in PC12
- VernacularTitle:RNA干扰技术对PC12细胞CaMKⅡ?基因表达的影响
- Author:
Jian-Gong LIU
;
Jiang-Tao WANG
;
Jun XIE
;
- Publication Type:Journal Article
- Keywords:
RNAi shRNA Gene expression CaMKⅡ
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effects of RNAi expression vector on CaMKⅡ? expression of PC12 cell lines. siRNA expression vectors were constructed to be targeted directly at CaMKⅡ? gene. The recombinants were transfected into PC12 cell line with liposome. The expression of CaMKⅡ? was detected by RT-PCR and Western blot. Expression vectors could reduce the expressions of CaMKⅡ? mRNA and protein in PC12, Compared with blank vector group, the former, the ratio of inhibition of the expression of CaMKⅡ? mRNA was 46.40% and 64.69%, in 48th hour and 72th hour, the ratio of inhibitory of the expression of CaMKⅡ? protein was 74.77% in 72th hour. RNAi expression vectors can effectively inhibit the expression of CaMKⅡ? in PC12.
