A New Quantitative Determination Method for the Biological Activity of Recombinant Human Ciliary Neurotrophic Factor
- VernacularTitle:定量测定重组人睫状神经营养因子活性的新方法
- Author:
Hu ZHOU
;
Yi WANG
;
Jun-Ming ZHU
;
- Publication Type:Journal Article
- Keywords:
Ciliary neurotrophic factor Acid phosphatase Neuron cells Biological activity
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new quantitative determination method for the biological activity of recombinant human ciliary neurotrophic factor. Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally. Result: rhCNTF could promote original era dorsal root neuron cells of chick embryo surviving,the livability of neuron cells was positively related to the amount of rhCNTF added to the culture. Conclusion: A quantitative determination method for the biological activity of rhCNTF was developed by testing the activity of acid phosphatase in neuron cells. Compared with the typical ways,this method was quantificational easily,repeatable better and with much fewer disturbance factors.
