Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells
- VernacularTitle:八氢番茄红素合酶基因对人参的遗传转化
- Author:
Yuan-Hua YU
;
Bai-Quan DU
;
Yong ZHANG
;
Li ZHANG
;
- Publication Type:Journal Article
- Keywords:
Agrobacterium tumefaciens psy gene Ginseng callus cells Genetic transformat
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Phytoene synthase is a rate-limiting enzyme in the pathway of carotenoid biosynthesis.To aim at establishing a transformation of Ginseng callus cells, elevating the nutritive value through encouraging the composition of corresponding carotenoid,taking Ginseng callus cells as acceptor,psy gene were transformed into cells via Agrobacterium-mediated. The factors affecting genetict ransformation were also investigated seperately from concentrations of A.tumefaciens, age of Ginseng callus cells, infection time and co-culture time. PCR,PCR-Southern and RT-PCR analysis from the transferred gene plants proved that psy gene has been transferred into Ginseng callus cells and can be expressed. The content of ?-carotene was increased by an average of 26 times.A base of improving and raising the contents of carotenoid in the Ginseng callus cell was established.
