Cloning of Full Genome and Genotyping of a Group A Human Rotavirus
- VernacularTitle:A组人轮状病毒全基因组克隆和基因型分析
- Author:
Yuan-Ding CHEN
;
Xiao LIU
;
Xin-Yu XIONG
;
Zhi-Liang CAO
;
Yu-Ling WEN
;
Qing-Huan ZHAO
;
Yang YU
;
Xing-Xiao YIN
;
Chuan-Yin LI
;
Yaochun FAN
;
- Publication Type:Journal Article
- Keywords:
Group A human rotavirus Full genome Cloning Genotyping analysis
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
By means of genetic cloning and recombinant techniques, full genome cDNA sequences of rotavirus strain TB-Chen were isolated from an infantile hospitalized with acute gastroenteritis. Nucleotide sequences analyses showed that the full genome of strain TB-Chen contains 18613 nucleotides, encoding 5791 amino acids. Genotyping results showed that the strain TB-Chen belongs to genotype G2P[4]/NSp4[A]. This is the first report on a full genome of Group A rotavirus in China, and has important significance for deep understanding structure and functions of rotaviruses and developing rotavirus vaccines.
