An Oxygen-tolerant Denitrifying Strain and Its Denitrifying Processes
- VernacularTitle:一株高耐氧反硝化细菌的筛选及其反硝化产物确定
- Author:
Fan HE
;
Yi-Jun KANG
;
Jun SHAN
;
Jian HU
;
Shi-Xue YIN
;
- Publication Type:Journal Article
- Keywords:
Denitrifying bacteria;
Denitrifying products;
Screen;
Oxygen-tolerant
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
An oxygen-tolerant denitrifying strain designated as H1 was screened by the procedures of shallow shaking and continuous aeration cultures.With the aid of an nnrS-gfp fusion responsive to nitric oxide (NO)and acetylene inhibition-GC procedure,it was shown that strain H1 was able to produce NO and N_2O but not N_2 under denitrifying conditions.Denitrifying processes were thus determined as NO_3~-→NO_2~-→NO→N_2O,with N_2O as the end product.Strain H1 could denitrify under shallow shaking conditions as well as in the initial atmospheric oxygen concentration ranging from 0～21%.Denitrification processed normally under continuous aeration at the rate of 2 L air per min in a 150 mL medium,but stopped under high aeration rate as 5 L air per min.16S rRNA gene sequence revealed that strain H1 shared 98% similarity to its closet relative Ralstonia taiwanensis,the genus where denitrifying bacteria are frequently found.
