Optimization of Saccharomyces cerevisiae sp.Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase
- VernacularTitle:酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化
- Author:
Fen YAN
;
Qian WANG
;
Zi-Lin LIN
;
Yang-Hao GUO
;
- Publication Type:Journal Article
- Keywords:
D-(-)-mandelate;
D-(-)-mandelate dehydrogenase;
Saccharomyces cerevisiae sp.,Fermentation condition
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The culture conditions of Saccharomyces cerevisiae sp.strain by 1.1 b were optimized for the production of D-(-)-mandelate dehydrogenase which is useful for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate.The optimum medium(per liter)consistes of 60 g peptone,30 g maltose, 0.5 g MgSO_4,0.01 g ZnSO_4,1.0 g KCl.After optimization of the culture medium,the enzyme production in shake flasks is enhanced from 2.56 to 20.21 U/L.The optimum fermentation conditions were determined as follows:medium volume 100 mL(i.e.,40%for a 250-mL shake flask),pH 6.5,inoculum size 10%,temperature 30℃,and cultivation time 25 h.
