A New Method of Soil Bacterial DNA Extraction and T-RFLP Analysis for Diversity
- VernacularTitle:土壤细菌DNA提取及多样性分析的T-RFLP方法
- Author:
Yun-Ying GE
;
Song CHEN
;
Lan HU
;
Zheng TU
;
- Publication Type:Journal Article
- Keywords:
Soil bacterial;
Diversity analysis;
DNA extraction;
T-RFLP
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study.A quick, efficient,sensitive and stably method of DNA extraction from soil were established by combining strongpoints of two kits(Soilmaster kit and DNA IQ~(TM)kit).In addition,the 16S rDNA gene and T-RFLP(Terminal restriction fragment length polymorphism)were used in the analysis of soil bacterial community diversity and the result show that T-RFLP is a powerful tool for bacterial community study.
