Determination of Dihydroxyacetone in Fermentation Broth by HPLC
- VernacularTitle:高效液相色谱测定发酵液中1,3-二羟基丙酮(DHA)方法的建立
- Author:
Zhi-Ming RAO
;
Mei-Juan XU
;
Wei SHEN
;
Hui-Ying FANG
;
Jian ZHUGE
;
- Publication Type:Journal Article
- Keywords:
Dihydroxyacetone HPLC Determination
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A method to determine dihydroxyacetone (DHA) in fermentation broth was developed by high performance liquid chromatography (HPLC). DHA was separated on a Alltima C18(5?m,250?4.6mm). The mobile phase was 0.5% methanol solution (pH adjusted to 3.0 with H3PO4), the flow-rate was 1.0 ml/min and the detective wavelength was 200 nm. The detection limits of DHA was 0.1 g/L～10.0 g/L. 6.2 g/L DHA in the fermentation broth was detected by HPLC, which was in agreement with the result by spectrophotometric method.The method was applicable for DHA determination in the fermentation process.
