Cloning and Expression of dauW Gene from Daunorubicin-producing Streptomyces coeruleorubidus SIPI-1482
- VernacularTitle:天蓝淡红链霉菌SIPI-1482的dauW基因的克隆及在大肠杆菌中的表达
- Author:
He HU
;
Ke SHANG
;
You-Jia HU
;
Bao-Quan ZHU
;
Chun-Bao ZHU
;
- Publication Type:Journal Article
- Keywords:
dauW Streptomyces coeruleorubidus Daunorubicin Self resistance
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
A novel gene,located between dnrX and drrB in the genome of daunorubicin-producing strain Streptomyces coeruleorubidus SIPI-1482,was cloned and named as dauW.The full sequence of dauW was submitted to GenBank(Accession No.EF523565).Blast result indicated that it showed high homology with dnrW in GenBank.The exact function of dauW is as yet unknown despite the possibility that it might belong to a family of FAD-dependent oxidoreductases on the basis of conserved domain analysis.dauW was cloned into expression plasmids pET-28a(+)and pET-32a(+),respectively,and was successfully expressed in E.coli DE3 after induction with IPTG.The preliminary results of the expression of dauW suggested that it might be involved in the self resistance in Streptomyces coeruleorubidus due to the increased resistance to daunorubicin in the E.coli host.
