Optimization of Culture Conditions for a Methane Utilizing Strain and Its Preliminary Application

VernacularTitle:甲烷利用菌培养条件的优化及其初步应用
Author: Jun ZHENG ; Hong-Bing CHENG ; Shang-Jun LIU ; Gen-Gui ZHAO ;
Publication Type:Journal Article
Keywords: Pseudomonas RSM Methane
From: China Biotechnology 2006;0(12):-
CountryChina
Language:Chinese
Abstract: Statistic-based experimental design was used to optimize the culture conditions for Pseudomonas aeruginosa ME16 strain which is capable of utilizing methane.Effects of inoculum volume,temperature,methane content,and initial pH of media on cell growth were studied with the medium of mineral salt and methane gas,and the response surface methodology(RSM) was then used to optimize culture conditions.The optimum conditions were as follows:temperature of 29.4℃,inoculum volume of 1.8% and methane content of 25%.Under the optimized culture conditions,the ME16 production was increased by 0.8 times and its cultivation time was reduced.The removal of methane gas by ME16 was preliminary investigated,and the removal rate of methane gas reached 65.7%.The results indicated that the strain was capable of removing of methane.