Studies of Temperature Shift Fermentation for Eicosapentaenoic Acid Production by Nitzschia laevis
- VernacularTitle:硅藻变温发酵生产二十碳五烯酸的研究
- Author:
Xiao-Hong CAO
;
Yu-Hua ZHAO
;
Mei-Fang LU
;
Jing LEI
;
Chun-Ling WANG
;
- Publication Type:Journal Article
- Keywords:
EPA Diatom Nitzschia laevis Temperature shift fermentation
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Fermentation for Eicosapentaenoic Acid(EPA) production by Nitzschia laevis at various temperature between 10℃ and 30℃ was investigated and the dynamics characteristics during fermentation process were also analyzed.Based on the results,a varying temperature nursing method of two stage control strategy is proposed:During the first stage,which comprises the delay phase and the initial index phase,the temperature is maintained at 25℃;then the temperature is shifted to 20℃ and kept up till the end of the fermentation process.By this method,a EPA content of 6.0% and a yield of 291.60 mg/L have been gained.These are 24.07% and 18.81% higher than that of fixed temperature(25℃) fermentation,respectively.
