Studies on the Production and Properties of Chitinase from Nomuraea rileyi Strain CQ031021
- VernacularTitle:莱氏野村菌产几丁质酶条件及酶学性质研究
- Author:
Yi ZHAI
;
Zeng TU
;
Jin-Fang WANG
;
Yong-Ji WAN
;
- Publication Type:Journal Article
- Keywords:
Nomuraea rileyi,Chitinase,Enzyme properties,Producing condition
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The producing condition and properties of chitinase secreted by Nomuraea rileyi strain CQ031021 were studied.The optimal conditions for the strain to produce chitinase are 6 days of 28℃ with the initial pH 6.0,and the liquid medium containing 2.0% glucose as its carbon source and 0.6% peptone plus 0.6% beef extract as nitrogen source after inoculating dosage 2mL suspension of conidia(1?107/mL).The optimal temperature and pH for enzyme activity is 50℃ and 6.0,respectively,while the activity can be enhanced by Tween-80 and inhibited by SDS.The enzyme activity is stable under 40℃ and in pH range of 5.5～6.5.
