Isolation and Characterization of a ?-tubulin Gene from Trichoderma Harzianum
- VernacularTitle:哈茨木霉?微管蛋白基因的克隆与序列分析
- Author:
Min LI
;
Qian YANG
;
- Publication Type:Journal Article
- Keywords:
Trichoderma harzianum,Inverse PCR,?-tubulin,Homology modeling
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on a ?-tubulin gene EST sequence from Trichoderma harzianum mycelium cDNA library,a 1.74kb ?-tubulin gene coding region,together with a 1.5kb 5'-flanking region and a 1.0kb 3'-flanking region was cloned from T.harzianum by IPCR methods.The gene encodes a 446 amino acid polypeptide,which shows a high degree of homology with other fungal ?-tubulins.A three-dimensional model of the T.harzianum ?-tubulin was built based on the crystal structure of bovine tubulin using homology modeling method.Tertiary protein structure of the ?-tubulin should provide a basis for understanding a significant body of research on tubulin's properties and interactions with antimicrotubule agents.
