Studies on Purification and Properties of Phytase from Trichoderma viride
- VernacularTitle:植酸酶的纯化及其酶学性质初步研究
- Author:
Yan-Ling YANG
;
Shi-Hua WANG
;
Kai-Hui HU
;
- Publication Type:Journal Article
- Keywords:
Phyatse,Purification,Enzymology
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The phytase was extracted from solid state leavening of Trichoderma Viride LH374.The crude product was purified by(NH_4)_2SO_4 precipitation,gel filtration and ion exchange chromatography.The purified phyatse was 13.3 times of the raw products,and the extraction ration was 27.1%.The study on the enzymology of phyatse showed that the optimal temperature and pH were 55℃ and 6.0,respectively.The Km value of the phytase was 0.15mmol/L.
