Construction and Screening of a Phage Display Library of Repertoire Single Chain Fv Antibody from Mouse Immunized with BoNTB/Hc
- VernacularTitle:鼠源抗B型肉毒毒素单链抗体噬菌体文库的构建筛选及抗体免疫学活性的初步研究
- Author:
Xiu-Qing YANG
;
Hui WANG
;
Jing SHI
;
Kun CAI
;
Xiao-Jun HOU
;
Shi-Zhong BAO
;
Jun YIN
;
- Publication Type:Journal Article
- Keywords:
Botulinum neurotoxin type B,scFv,Phage display library
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To produce antibodies capable of neutralizing botulinum neurotoxin type B(BoNT/B),We cloned the carboxy-terminal end of Hc containing the major determinants responsible for specific toxin,induced and purifed.The heavy-chain and kappa light-chain variable region gene repertoire of immunoglobulin were amplified individually from the spleen cell mRNA by RT-PCR and joined as a single-chain Fv(scFv)DNA fragment.These fragment were cloned into the phagemid pCANTAB5E and the phage display library was constructed.Results showed that the high affinity scFv was obtained after 4 rounds of panning,with its DNA sequence conforming to that of mouse antibody.
