The molecular epidemiology of methicillin-resistant Staphylococcus aureus in China in 2005
- VernacularTitle:2005年我国多中心苯唑西林耐药的金黄色葡萄球菌分子流行病学研究
- Author:
Hui WANG
;
Yu-Dong LIU
;
Na DU
;
Hong-Li SUN
;
Ying-Chun XU
;
Min-Jun CHEN
;
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
Epidemiology,molecular;
Sequence analysis,DNA;
Oxacillin;
Drug resistance,bacterial
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiology of methicillin-resistant Staphylococcus aureus in China in 2005.Methods From January to December 2005,395 consecutive and non-repetitive isolates of methicillin-resistant Staphylococcus aureus were collected from 17 teaching hospitals in 14 cities.The genotypes of SCCmec were determined by multiplex PCR pulsed-field gel electrophoresis (PFGE)was used to type the chromosome DNA of MRSA.Muhilocus sequence typing(MLST)was used to type the housekeeping genes.Fifty-three strains were selected for MLST typing according to the antimicrobials susceptibility patterns,PFGE types,SCCmec types and the distribution of the regions.The toxin gene was detected by PCR.Results Among 395 isolates of MRSA,SCCmec Ⅲ,untypeable type and type Ⅱ accounted for 61.5%(243/395),24.3%(96/395)and 14.2%(56/395)respectively.In Shenyang,60.7%(17/28)of the isolates were SCCmec Ⅱ,which was significantly higher than other areas. Twenty-four different types and 42 subtypes were found by PFGE typing.Clone A accounted for 50.1%, existing in 13 teaching hospitals in 12 cities and clone R accounted for 23.5%,existing in 9 teaching hospitals in 8 cities.Six sequence types(ST)were found in these isolates with ST239 and ST5 accounting for 75.5% and 17.0% among these isolates,respectively.The prevalence of pvl gene was 2.5% among 395 isolates of MRSA.Conclusions The most types of SCCmec in China were Ⅲ and Ⅱ,and distribution of SCCmec types differed among regions.MRSA outbreaks caused by epidemic multiple-drug resistant clones occurred in big teaching hospitals in China.Meanwhile,the same PFGE pattern may spread among areas. Several international epidemic MRSA clones may exist in China.
