Analysis of energy metabolism related genes in human myocardial cell by cDNA microarray
- VernacularTitle:cDNA基因芯片分析人心肌细胞能量代谢相关基因
- Author:
Man ZHANG
;
Jing WANG
;
Shan CHI
;
- Publication Type:Journal Article
- Keywords:
Genes;
Microarray;
Myocardium
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective We selected 3 268 genes of human myocardial cells and analyzed the variability of mRNA expression of myocardial cells in normal adults,to explore the variation of energy metabolism related genes in human myocardial cell and compared the characteristics and variation tendency of energy metabolism related genes in human myocardial cells in the early stage.Methods Microarray was prepared with 3 368 myocardial cell related cDNAs.And we studied differentially expressed genes between myocardial cells and multi-tissues in normal adults by PCR,sequence,fluorescent probe labelling and double hybridisation.Results Functional group analysis was carried out with 3 268 genes of myocardial cells in normal adults.Compared with Universal Human Reference,576 genes from myocardial cells were up- regulated(≥1.5 folds).Expression of 1 199 genes were down-regulated(≤66.7%).194 genes were involved in cell energy metabolism.Among them,17 genes were up-regulated and 4 genes were down- regulated.65 genes were related with cellular skeleton,18 of them had up-regulation and one of them had down-regulation.Coneluslon Compared with multi-tissues,there were many genes preferentially expressed in myocardial cells of normal adults,which can be used as a reference system to evaluate the different stages and different pathological behaviors of cardiovascular diseases.
