Co-Immobilization of Permeabilized Meiothermus sp.Cells Containing Trehalose Synthase
- VernacularTitle:亚栖热菌透性化细胞的耦合固定化研究
- Author:
Jun ZHANG
;
Ying CHEN
;
Xiao-Yun CHEN
;
Xin QI
;
Xue-Sheng WEI
;
- Publication Type:Journal Article
- Keywords:
Meiothermus sp. Trehalose synthase Co-immobilization
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Permeabilized cells containing trehalose synthase from Meiothermus sp.CBS-01 was immobilized by the integration of entrapment in alginate beads with cross-linking and electrostatic self-assembly coating technique.Coating on alginate beads with diazo-resin and poly(styrene sulfonate) can protect the beads from degradation in phosphate buffer,whereas cross-linking with carbodiimide improves the thermostability of trehalose synthase entrapped in alginate beads.By co-immobilization of permeabilized cells using entrapment-cross-linking- coating method,the enzyme recovery was 32%,and the optimum temperature of the enzyme was still 60℃,but the optimum pH was shifted from 6.5 to 7.In batch manner,a maximum conversion yield of 60% trehalose from maltose could be reached by the immobilized cells.Within 4 times repeated use of the immobilized cells,and each time was operated at 50℃ for 24h,more than 50% conversion yield of trehalose could be maintained with less than 20% loss of the enzyme activity.
