Application of Element and Metabolism Balancing in the Production Phase of Human-like Collagen
- VernacularTitle:元素衡算和代谢衡算在类人胶原蛋白表达期的应用研究
- Author:
Yan-E LUO
;
Dai-Di FAN
;
Xiao-Xuan MA
;
- Publication Type:Journal Article
- Keywords:
Element balancing Metabolism balancing Recombinant Escherichia coli BL21 Human-like collagen
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
On the base of element and metabolism balancing,the mathematic model of the human-like collagen expression phase with recombinant Escherichia coli BL21 was developed and the unknown parameters in the model were estimated with the method of nonlinear optimization.The model was in agreement with the growth kinetics and the metabolic kinetics,and the key calculated parameters of ?h,?p and mx were 1.173 mol?C-mol-1,293.814 mol?C-mol-1 and 17.878 mol?C-mol-1?h-1 respectively.This model could preferably predict the macroscopic reaction rates,and in the synthesis phase of human-like collagen,the specific growth rate should be controlled at 0.04 h-1 with controlling glucose feeding rate to gain the highest specific production rate of human-like collagen.
